Which of the following is least likely to interfere with hemoglobin measurements?

Cold agglutinins are antibodies that can cause erythrocyte agglutination at lower temperatures, but they have a limited effect on hemoglobin measurements. Hemoglobin tests typically measure the amount of hemoglobin present in the blood and are usually conducted at body temperature, where the agglutination caused by cold agglutinins is minimized. Therefore, their impact on the accuracy of hemoglobin quantification is significantly lower compared to the other options listed.

High bilirubin levels can lead to the formation of methemoglobin or interfere with spectrophotometric readings, affecting the accuracy of hemoglobin assessment. Lipemia can cause turbidity in the sample, obscuring measurements and potentially leading to false results. Hemolysis, the breakdown of red blood cells, releases hemoglobin into the plasma, which can artificially elevate hemoglobin levels in serum/plasma measurements and alter results dramatically.

In contrast, cold agglutinins do not pose significant interference during the standard hemoglobin measurement process, making them the least likely to affect the accuracy of these tests.