What is the primary use of 8-Hydroxyquinoline and CPC reagent?

8-Hydroxyquinoline and CPC (cetylpyridinium chloride) reagent are primarily utilized in the determination of calcium in laboratory settings. The mechanism involves the formation of a complex between calcium ions and 8-hydroxyquinoline, which can then be quantitatively measured. This complex formation is crucial in providing a sensitive and specific method for calcium analysis, allowing laboratory technicians to accurately assess calcium levels in clinical samples.

In the context of clinical chemistry, the ability to effectively measure calcium is essential, as it plays a significant role in various physiological processes, including bone health, muscle function, and nerve signaling. The utilization of specific reagents like 8-hydroxyquinoline and CPC enhances the reliability of calcium measurements, making this method widely accepted and used in laboratories for accurate potassium analysis.